A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue or ...

Western blotting (or immunoblotting) is a well-established biochemical technique that detects specific proteins in complex samples and has applications from basic research to the diagnosis of ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

This article describes the histone western blot protocol, which is often used at Abcam for identifying histone proteins obtained from purified calf thymus. For each lane, either 0.5 μg of calf thymus ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

In this editorial, Kenneth Oh overviews the two main normalization methods used to quantify western blots and discusses common errors to avoid. What is western blot normalization? Kenneth Oh is the ...

Western blotting is an important technique for characterizing specific protein targets. Successful western detection is dependent on the quality of protein transfer from polyacrylamide gels to the ...

Western blotting is a dependable, trusted method for protein detection used by thousands of labs worldwide. While the process of western blotting often seems straightforward, different variables used ...

The western blotting or protein blotting process was originally developed in 1979. This process has become a powerful and popular technique for the visualization and identification of proteins. The ...